Journal: Research in Veterinary Science

Article Title: Relationship between rate of infection and markers of inflammation/immunity in Holy Birman cats with feline coronavirus

doi: 10.1016/j.rvsc.2014.08.009

Figure Lengend Snippet: Results recorded in Birman cats and in cats from other breeds.

Article Snippet: Immunophenotyping by flow cytometry was performed on aliquots of 50 μL of the cell suspension to identify lymphocyte subpopulations as previously described ( ) using the following panel of antibodies for feline surface antigen: 2.5 μL of mouse anti feline CD4 (specific for T helper cells, clone MCA1350, Serotec, Oxford, UK), 1 μL of mouse anti feline CD8 alpha/beta (specific for T cytotoxic cells, clone MCA1347G, Serotec, Oxford, UK), 50 μL of mouse anti feline CD5 (specific for T cells, clone MCA2038S, Serotec, Oxford, UK), and 1 μL of mouse anti canine CD21, specific for B cells, that cross reacts with feline species (clone MCA1781R, Serotec).

Techniques:

Journal: Journal of Veterinary Internal Medicine

Article Title: Pharmacokinetics of mycophenolic acid and its effect on CD4 + and CD8 + T cells after oral administration of mycophenolate mofetil to healthy cats

doi: 10.1111/jvim.15585

Figure Lengend Snippet: CD4 + /CD8 + ratios (mean ± standard deviation) for all cats (n = 10) given different doses of MMF on days 1, 2, 7, 8

Article Snippet: Cells in the 2 aliquots (100 μL/aliquot) were pelleted by centrifugation at 1800 g for 8 minutes and incubated with 100 μL of PBS solution and optimal concentrations of fluorescein isothiocyanate‐conjugated CD4 + (Mouse anti‐cat CD4:FITC, Bio‐Rad, Hercules, California) and phycoerythrin‐conjugated CD8 + (Mouse anti‐cat CD8 alpha/beta:RPE, Bio‐Rad) for 15 minutes at 4°C in the dark.

Techniques: Standard Deviation

Journal: International journal of molecular sciences

Article Title: Embedded Human Periodontal Ligament Stem Cells Spheroids Enhance Cementogenic Differentiation via Plasminogen Activator Inhibitor 1.

doi: 10.3390/ijms23042340

Figure Lengend Snippet: Figure 1. The effect of rhPAI-1 on cementogenic differentiation of HPLSCs in 2D culture. (A) Western blot of cementum protein 1 (CEMP1) and cementum attachment protein (CAP) in HPLSCs that were cultured in 2D with DMEM (control), osteogenic inducing medium (OIM) alone, and OIM containing rhPAI-1 (PAI-1; 100 ng/mL) for 3 weeks using β-actin as a loading control. (B) Quantification of the protein expression was performed using the ImageQuant LAS4000 biomolecular imager. The relative levels of CEMP1 and CAP expression were normalized to β-actin and were expressed as fold changes compared with HPLSCs that were cultured with DMEM (control). Data represent the mean ± SD of three independent experiments. n.s., not significant, * p < 0.01, ** p < 0.05. (C) Fluorescence images of the HPLSCs that were stained with CEMP1 (red) and CAP (red) in 2D culture with DMEM (control), OIM alone (OIM), and OIM containing rhPAI-1 (100 ng/mL) for 3 weeks. The nuclei were counterstained with DAPI (blue). The scale bar represents 20 µm. (D) Semiquantification of the percentage of positive cells that were stained with CEMP1 (nuclei) and CAP (perinuclear cytoplasm). Data represent the mean ± SD of three independent experiments. n.s., not significant, * p < 0.001. (E) Staining for ALP activity in the HPLSCs in 2D culture with OIM alone (OIM) or with PAI-1 for 3 weeks. Alizarin red-staining (ARS) was also performed in the cells that were cultured with OIM alone or with PAI-1 for 3 weeks. (F) Semiquantification of the staining intensities of ALP and ARS using the WinROOF image analysis software. The relative staining intensities of both ALP and ARS of HPLSCs that were cultured with OIM were expressed as fold changes. Data are displayed as the mean ± SD of three independent experiments. * p < 0.001, ** p < 0.05.

Article Snippet: The mouse anti-actin beta (β-actin) antibody (×1500: VMA00048, Bio-Rad Laboratories, Hercules, CA, USA) was used as a loading control.

Techniques: Western Blot, Cell Culture, Control, Expressing, Fluorescence, Staining, Activity Assay, Software